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To answer this question, researchers begin by stating a
                      null hypothesis   , which predicts that the intervention or   100

                      treatment has no effect at all. In other words, any differ-
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                      ence between the test and control groups is due to chance   90
                      alone and nothing else is responsible for the difference
                      between the two groups. In this case, the null hypothesis is  Average heart rate (beats per minute)  80  Average heart
                      that  caffeine does not cause an increase in heart rate. They   rate of students  Error bar

                      also state an  alternative hypothesis   ,  which predicts that   70 after 0 cups
                                                                                    of coffee
                      the intervention or treatment has an effect, so the difference   60
                      between the test and control groups is real. In this case, the
                      alternative hypothesis is that caffeine causes an increase in    0       1       2      3       4
                                                                                                  Cups of co ee
                      resting heart rate.
                            A statistical test normally yields a number, called the   FIGURE   0.8        Error bars
                        p -value, that expresses the likelihood that an observed result   An error bar represents a range of values within which the true value
                      could have been observed merely by chance. A  p -value is a   is likely to be. This graph shows the relationship between caffeine

                                               ,

                      probability. If  p ≤ 0.05 (5%)  there is less than or equal to a   consumption and resting heart rate. It plots the average resting
                      5% chance that the observed results are the result of chance.   heart rate of groups of people who consumed 0, 1, 2, 3, or 4 cups
                                                                              of coffee, with error bars giving an indication of the uncertainty of
                      This is a relatively small chance. In this case, it is likely that   the data.
                      the observed results in a dataset are real and not due to
                      chance. In other words, the null hypothesis is rejected. By   As a result, you can think of the error bar as a way to show


                                       ,
                      contrast, if  p > 0.05  there is greater than a 5% chance that   the uncertainty of a measurement or data point.
                      the observed results could have been obtained by chance, so     Throughout this course, you will have the opportunity
                      you fail to reject the null hypothesis. The phrasing “the null   to learn some of the techniques researchers use to evaluate
                      hypothesis has failed to be rejected” reminds us that although   data and to work with data yourself. “Tutorial 1:  Statistics”
                      the results do not support the alternative hypothesis, it does   on page 20 walks you through some of the statistics tools
                      not mean that the null hypothesis is correct. Rather, it sim-  you will encounter. “Analyzing Statistics and Data:  Averages”
                      ply means that the null hypothesis has not been disproven.   gives you a chance to practice working with these concepts.
                            Finally, scientists are often interested in determining
                      how confident they should be in their data. Uncertainty can
                      be shown graphically as an error bar. An error bar is typically
                                                                                                                         ®
                                                                                                                     PREP FOR THE AP  EXAM
                      a short vertical line showing a range of values. For  example,   ®

                       FIGURE 0.8   shows results from our controlled experiment      AP   EXAM TIP
                      investigating the relationship between caffeine and resting     When evaluating the results of an experiment, you should
                      heart rate. The data points indicate the average heart rate of   be able to explain whether the alternative hypothesis is
                      the people in the groups. The vertical lines through the data   supported or not, and whether the null hypothesis should
                      points are error bars. In spite of its name, an error bar doesn’t   be rejected or fail to be rejected. you should know that
                                                                                 data are often analyzed statistically to determine if real
                      represent an error or a mistake. Instead, it shows a range of   differences exist between the data collected from the
                      values that incorporates small differences among the indi-  experimental and control groups.
                      viduals and perhaps even inaccuracies in the measurements.


















                                                                                                      MODULE 0   IntroductIon   11

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          02_morrisapbiology1e_11331_Unit1_Mod0_01-19_3pp.indd   11                                                             10/04/21   9:09 AM
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